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GitHub - sc932/ALE: Assembly Likelihood Estimator
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Assembly Likelihood Estimator
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sc932/ALE
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DOCUMENTATION ------------- https://portal.nersc.gov/dna/RD/Adv-Seq/ALE-doc/ This code is no longer in active development. Pull requests and bugs will be addressed as needed. Plotting -------- The authors recommend using IGV to view the output. https://www.broadinstitute.org/igv/ Just import the assembly, bam and ALE scores. You can convert the .ale file to a set of .wig files with ale2wiggle.py and IGV can read those directly. Depending on your genome size you may want to convert the .wig files to the BigWig format. LICENSE ------- /* * Copyright (C) 2010,2011,2012 Scott Clark. All rights reserved. * * Developed by: * Scott Clark * Cornell University Center for Applied Mathematics * https://cam.cornell.edu * AND * Rob Egan * Department of Energy Joint Genome Institute * https://jgi.doe.gov * * Permission is hereby granted, free of charge, to any person obtaining a * copy of this software and associated documentation files (the "Software"), * to deal with the Software without restriction, including without limitation * the rights to use, copy, modify, merge, publish, distribute, sublicense, * and/or sell copies of the Software, and to permit persons to whom the * Software is furnished to do so, subject to the following conditions: * * 1. Redistributions of source code must retain the above copyright notice, * this list of conditions and the following disclaimers. * 2. Redistributions in binary form must reproduce the above copyright * notice, this list of conditions and the following disclaimers in the * documentation and/or other materials provided with the distribution. * 3. Neither the names of Cornell University, The Joint Genome Institute, * nor the names of its contributors may be used to endorse or promote * products derived from this Software without specific prior written * permission. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER * DEALINGS WITH THE SOFTWARE. */ // For more information on the licence please see // The University of Illinois/NCSA Open Source License // https://www.opensource.org/licenses/UoI-NCSA.php INSTALL ------- https://portal.nersc.gov/dna/RD/Adv-Seq/ALE-doc/index.html#installation # # ALE comes packaged with samtools-0.1.18 Feel free to use a later version # samtools is open source (MIT license) # We would like to thank the creators for the extensive and very useful package # Requirements for plotter4.py: numpy 1.6.1 mpmath 0.17 setuptools 0.6c11 matplotlib 1.0.1 pymix 0.8 https://www.pymix.org/pymix/
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