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Welcome to FocalPlane

FocalPlane is a community site for anyone who uses microscopy in their research. It is a place where you can interact with a global community of imaging scientists, engineers, chemists, and bioimage analysts.

FocalPlane is your site and once registered, you are free to share a blog post, add an image to our gallery, post a job advert or put up an event listing.

Create an account to share your story or to stay up-to-date with the latest news.

Join or access here

Recent posts

Microscopy preprints – applications in biology

Posted by FocalPlane, on 23 January 2026

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

Imaris – 3D image visualization and analysis course report

Posted by Quentin LEBEL, on 23 January 2026

I attended a training session at the Bordeaux Faculty to learn how to use IMARIS, a software designed for viewing, measuring, and analyzing 3D microscopy images. The training, organized by the Bordeaux Imaging Center (BIC), was structured to first provide an overview of the software’s broad capabilities and then dig into more specific applications tailored

My participation at the GloBIAS Conference 2025 in Kobe Japan

Posted by Beatriz Serrano-Solano, on 22 January 2026

This article was written by Dr Libert Brice Tonfack, Associate Professor in Plant Biotechnologies at the University of Yaounde I, Cameroon. Upon returning from my active participation in the first conference organized by the Global Bioimage Analysts’ Society (GloBIAS) in 2024 in Gothenburg, Sweden, I immediately understood that I needed to commit strongly to the

Imaging spotlight: visualising synaptic vesicle fusion by cryo-ET

Posted by FocalPlane, on 21 January 2026

In this paper highlight from Jana Kroll and colleagues, we hear about their research combing optogenetic stimulation with plunge freezing and cryo-electron tomography to study synaptic vesicle fusion.

Imaging spotlight: U-ExM atlas of microbial eukaryotes

Posted by FocalPlane, on 20 January 2026

In this paper highlight from Hiral Shah and colleagues, we learn about their ultrastructure expansion microscopy (U-ExM) atlas of over 200 cultured planktonic eukaryotes. This post includes technical advice for ExM users and highlights the open-access resource that they have generated for cytoskeleton or marine microorganism aficionados (and microscopy sciart lovers!)

Cytoneme Imaging Tips and Tricks

Posted by Christina Daly, on 19 January 2026

There has been a growing interest in visualizing long and thin membrane extensions. The field of intercellular connections has exploded, with elegant bioimaging studies focusing on structures like cytonemes, tunneling nanotubes (TNTs), intercellular/cytokinetic bridges (IB/CBs), and migrasomes. This enhanced interest in the intercellular communication facilitated by these extensions is largely due to the recent technological

Imaging spotlight: Initiation of chromosome congression deconstructed by multi-scale live lattice light-sheet and super-resolution microscopy

Posted by Kruno Vukušić, on 16 January 2026

In this highlight, Kruno Vukušić and Iva M. Tolić describe how they use multi-scale live lattice light-sheet and super-resolution microscopy to decipher the earliest steps of chromosome congression.

View more posts …

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Image credits

Neuromuscular junctions – Rebecca Simkins

Mitochondria and microtubules – Till Stephan

Mammary gland organoid – Oona Paavolainen

Dopaminergic neuron – Nick Gatford

CLEM HeLa cell – Marie-Charlotte Domart, Chris Peddie

Posts by categories

Microscopy-related articles from our journals

Toggle-Untoggle – a cell segmentation tool with an interactive user verification interface J Cell Sci 2025 138: jcs264154
The ratio of Wnt signaling activity to Sox2 transcription factor levels predicts neuromesodermal fate potential Development 2025 152: dev204661
The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity Biology Open 2025 14: bio062117

Microscopy-related preprint highlights

When geometry isn’t enough, actin helps plant cells decide where to divide, ensuring robust tissue patterning
Planar cell polarity protein, Vangl2, goes out of its way to shape the heart through a planar polarity-independent mechanism.
HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton

From the gallery

A ‘Cell-estial Bloom’ of human induced pluripotent stem cells.

View the gallery

Gallery categories

Bioimage analysisApply
WidefieldApply
Confocal microscopyApply
Spinning disc micros..copyApply
Super-resolution mic..roscopyApply
Single plane illumin..ation microscopy (SPIM / Light sheet)Apply
Total internal refle..ction fluorescence (TIRF) microscopyApply
Electron microscopyApply
Multi-photon microsc..opyApply
Label-free microscop..yApply
DyesApply
Genetic probesApply
Optical manipulationApply
Functional imagingApply

Updated on 12 December 2025

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