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An automated bioreactor for rapid growth and directed evolution of genetically modified bacteria strains. Applicable in the study of synthesis of biofuels and other organics in modified bacteria.
The code assumes the existence of a single reactor, hence numerous global
variables (reactor of class Reactor, db as an sqlite3 connection
object, calibration as a json dict, etc.) spread over submodules
(reactor, database, calibration, etc.). The number of wells is hard
coded (maybe it will move to calibration in the distant future).
Three threads are created: the scheduler from submodule scheduler; the web
interface from submodule web; the temperature control in reactor. The web
interface talks to the scheduler from a single location. The scheduler does
not talk to anybody.
An sqlite on-disk database is used by most threads. Threads access the
database for reading and writing, relying only on sqlite's internal locks.
No optimizations of disk access are done (might lead to wear of flash-based
drives).
The templating for the web UI is rudimentary, relying only on str.format.
The navigation toolbar is hardcoded.
Not much attention is paid to encoding url parameters. Non ASCII parameters
are not guaranteed to work. Special symbols might explode. More testing
necessary.
Temperature control is done with a PID loop in a separate (third) thread.
Some protection and resets through usbdevicesfs is enabled (requires the
compilation of usbreset.c) in the case of a hangup. Additional watchdogs
are possible in the Arduino, but are not currently enabled.
Proper database normalization would be to have a single table with data
measurements with a column dedicated to measurement type, but the more naive
approach with multiple tables (one per measurement type) is good enough for
this simple project.
Copyright (C) 2017 Hegarty, Krastanov, Racharaks
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An automated bioreactor for rapid growth and directed evolution of genetically modified bacteria strains. Applicable in the study of synthesis of biofuels and other organics in modified bacteria.